Induction of rapid and selective cell necrosis in drosophila. Drummond nanoject ii autonanoliter injector nanoject ii autonanoliter injector pipets, pipettes and pipette tips. The nanoject ii auto nanoliter injector offers significant options and controls ideal for ultradelicate work with oocytes, embryos, and tissues. Drummond scientific 3000203gx replacement glass capillaries for nanoject ii autonanoliter injector. The tecan genesis workstation 150 is a multiple task liquid handling system that combines a system of microprocessorcontrolled liquid handling and other components to one instrument. With a few taps on the touchscreen, users can program the stateoftheart unit for efficient single manual injections or multiple injection cycle recipes. Role of a tachykininrelated peptide and its receptor in. Nanoject ii autonanoliter injector, on most micromanipulators the drummond nanoject ii. Systemscircuits arestriataltyrosinehydroxylaseinterneurons. Select an injection timetable and pressure range that suits individual needs and receive results that are dependable. The nanoject ii autonanoliter injector performs ultradelicate nanoliter injection procedures into oocytes, embryos, and tissues. Novel aquaporin 3 in killifish fundulus heteroclitus is not. The mixture was injected into the body cavity of the 5dayold adults between the second and third abdominal segment using a nanoject ii autonanoliter injector drummond scientific, broomall, pa. If you are viewing this page as a nonregistered user, the prices displayed is list price.
Drummond scientific 3000025sb support base for nanoject. Each nanoject ii autonanoliter injector comes complete with 200 borosilicate capillaries that can be pulled to the required tolerance together with a. Drummond scientific 3000025sb support base for nanoject ii auto nanoliter injector. Drummond nanoject ii autonanoliter injector, 110v, universal adapter, 2 vials of 100 capillary glass included condition. The dsrnas were diluted with phosphatebuffered saline pbs, ph 7. Nanoject ii autonanoliter injector drummond scientific. The drummond nanoject ii auto nanoliter injector is specifically designed to perform ultradelicate nanoliter injection procedures into cells. After 3 days incubation at 18c, oocytes were tested for their ability to transport water, urea, or glycerol. Drummond nanoject ii autonanoliter injector nanoject ii auto. Drummond scientific company mounted on an mp285 micromanipulator sutter instru. The new drummond programmable nanoject iii nanoliter injector offers a number of stateoftheart features to provide easy setup and operation as well as more. Pain inhibits grpr neurons via gabaergic signaling in the.
Nanoliter injector nano injector oocyte micro injector wpi. Damage to the wire plunger will result and injection volumes will not be accurate. In this protocol, ka administration is combined with in vivo recording of neuronal activity with wired and wireless. Following the injections, larvae were reared on an artificial diet under conditions described above until they pupated and their phenotypes were observed daily under a leica m165c microscope. Nanoject ii auto nanoliter injector at thomas scientific. Each nanoject ii autonanoliter injector comes complete with 200 borosilicate capillaries that can be pulled to the required tolerance together with a back filling needle and maintenance kit. Automated microprocessor controlled microinjection pipet delivering precise nanoliter volumes accurately. Nanoject ii autonanoliter injector from drummond scientific.
Deciphering the function of octopaminergic signaling on. Drummond nanoject ii autonanoliter injector fisher scientific. Ensure small dosages are reproducible and accurate with the programmable microinjectors. Each nanoject ii auto nanoliter injector comes complete with 300 borosilicate capillaries that can be pulled to the required tolerance together with a back filling needle and maintenance kit. For all experiments involving bacterial injection, a nanoject ii autonanoliter injector drummond scientific company, broomall, pa, usa was used to inject 69 nl of the following. Replacements for nanojet ii autonanoliter injector. Nanoliter injector microinjection system from harvard apparatus. Automated microprocessor controlled microinjection pipet delivering precise. Nanoject ii autonanoliter injector drummond sci youtube. Drummond replacement capillaries for nanojet ii injectors. Mar 29, 2017 lack of scientific reproducibility is a growing concern and weak experimental practices may contribute to irreproducibility. Here, we describe an optimized and versatile protocol for stereotaxic intrahippocampal administration of kainic acid ka in mice with a c57bl6 background. Nanoject ii autonanoliter injector drummond scienti. This products resides on a fisher scientific gsa or va contract.
The drummond scientific nanoject ii auto nanoliter injector is specifically designed to perform ultradelicate nanoliter injection procedures in oocytes, embryos. The drummond nanoject ii autonanoliter injector aids in extracellular amplification with the ability to record neural activity more accurately. Drummond scientific 3000203gxl replacement glass capillaries for nanoject ii auto nanoliter injector, 7 length pack of 100. The nanoject ii autonanoliter injector performs ultradelicate nanoliter injection procedures into. The drummond nanoject ii autonanoliter injector is specifically designed to. Manuals billing question software download sds safety data sheets product. Wing discs were dissected 6 h after injection and stained with pi, or flies were maintained at 25 c until adult hatching. Nanoject ii autonanoliter injector from drummond scientific co. Bap1 regulates epigenetic switch from pluripotency to.
Function of the natalisin receptor in mating of the oriental. Repeat the injection on a dozen oocytes for each mrna. Two chitin biosynthesis pathway genes in bactrocera dorsalis. The mixture was injected into the body cavity of the 3, 5 and 7 day oldadults directly between the second and third abdominal segments using a nanoject ii autonanoliter injector drummond scientific, broomall, pa. Mri compatible syringe pump chemyx product details. Patch clamp and perfusion techniques for studying ion. Nanoject ii autonanoliter injector krackeler scientific. Nanoject iii programmable nanoliter injector drummond sci. A standardized protocol for stereotaxic intrahippocampal. Automated microprocessor controlled microinjection pipet delivering precise nanoliter. Drummond laboratory injector automated nanoject ii youtube.
The new drummond programmable nanoject iii nanoliter injector offers a number of stateoftheart features to provide easy setup and operation as well as more accurate injection volumes down to 0. Drummond scientific 3000026 foot switch for nanoject ii, 4 w x 1. Anopheles gambiae larvae mount stronger immune responses. Each nanoject ii autonanoliter injector comes complete with 300 borosilicate capillaries that can be pulled to the required tolerance together with a back filling needle and maintenance kit. Selected oocytes were injected with 25 ng crna of sglt1, sglt3 and mutants, respectively using a nanoject ii auto nanoliter injector drummond scientific company, broomall, usa. Drummond nanoject ii autonanoliter injector available on gsava contract for federal government customers only. Each nanoject ii autonanoliter injector comes complete with 200 borosilicate capillaries. The drummond nanoject ii autonanoliter injector is specifically designed to perform ultradelicate nanoliter injection procedures into oocytes, embryos, and. Pipet accessories drummond nanoject ii autonanoliter injector nanoject ii autonanoliter fisher scientific.
Pdf patchclamp and perfusion techniques to study ion. Small interfering rna pathway modulates persistent infection. Mar 29, 2016 the nanoject ii autonanoliter injector, manufactured by drummond scientific company, is particularly devised for performing extremely delicate nanoliter injection procedurec into oocytes. Handheld drill kopf model 1474220 with matching drill. The nanoject ii autonanoliter injector offers significant options and controls ideal for ultradelicate work with oocytes, embryos, and tissues. Use the continuous flow function for a bubble pressure test. Sep 18, 2019 at the desired developmental stage, embryos were injected with a nanoject ii autonanoliter injector drummond scientific inc. Divergent molecular evolution in glutathione stransferase. One of the unique features of the nanoject ii is the use of a higher torque motor than in previous models. Drummond nanoject ii autonanoliter injector nanoject ii. The drummond nanoject ii autonanoliter injector is specifically designed to perform ultradelicate nanoliter injection procedures into cells.
Underwater firearms by yoel future war stories future war stories. The nanoject ii auto nanoliter injector performs ultradelicate nanoliter injection procedures into oocytes, embryos, and tissues. Each nanoject ii auto nanoliter injector comes complete with 200 borosilicate capillaries that can be pulled to the required tolerance together with a back filling needle and maintenance kit. For heterologous expression of transporters oocytes were incubated for 4 to 5 days at 16c in nd96 solution containing gentamycin. Drummond scientific 3000025sb support base for nanoject ii. Nanoject ii autonanoliter injector micromanipulator. Oct 25, 2015 each nanoject ii auto nanoliter injector comes complete with 200 borosilicate capillaries that can be pulled to the required tolerance together with a back filling needle and maintenance kit. Features of the drummond scientific nanoject ii autonanoliter injector include. Nanoject iii programmable nanoliter injector drummond scientific. Functional analysis of a triplet deletion in the gene. Nanoject ii microinjectors, drummond scientific vwr. Automated microprocessor controlled microinjection pipet delivering precise nanoliter volumes accurately remote control and nonrotating plunger eliminates vibration allowing precise injection minimizing possible cell rupture from microinjector selectable injection volumes.
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